Heterogeneity of midbrain dopamine neurons
Unique properties of mesoprefrontal neurons within a dual mesocorticolimbic dopamine system
Lammel S, Hetzel A, Hackel O, Jones I, Liss B, Roeper J
Here, we report the identification of a type of dopaminergic neuron within the mesocorticolimbic dopamine system with unconventional fast-firing properties and small DAT/TH mRNA expression ratios that selectively projects to prefrontal cortex and nucleus accumbens core and medial shell as well as to basolateral amygdala. In contrast, well-described conventional slow-firing dopamine midbrain neurons only project to the lateral shell of the nucleus accumbens and the dorsolateral striatum. Among this dual dopamine midbrain system defined in this study by converging anatomical, electrophysiological, and molecular properties, mesoprefrontal dopaminergic neurons are unique, as only they do not possess functional somatodendritic Girk2-coupled dopamine D2 autoreceptors.I am curious to know whether mesoprefrontal DA neurons are not inhibited by apomorphine, which has been the criteria for identifying DA neurons recorded in vivo.
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3 comments:
Well, mesocortical dopaminergic neurons are not inhibited by application of quinpirole (a D2 receptor agonist) in brain slices. Thus, I would assume that these cells were also not affected by apomorphine, which is a D1/D2 receptor agonist.
Thank you. This is really beautiful work!
Any thought on how to identify DA neurons in behaving animals with extracellular recording?
Thank you.
Maybe this paper can help you:
Robinson et al., 2004 (PNAS):
"Firing properties of dopamine neurons in freely moving dopamine-deficient mice: effects of dopamine receptor activation and anesthesia".
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